A new infrared analysis shows a critical roll of a Cu site of cytochrome c oxidase for the energy-coupling
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چکیده
Background: Cytochrome c oxidase reduces O2 coupled with proton-pumping. Results: A newly developed time-resolved infrared system reveals transient conformational changes in the proton-pumping pathway upon CO-binding to CuB in the O2 reduction site. Conclusion: CuB promotes proton collection and effective blockage of back-leak of pumping-protons. Significance: These critical findings in Bioenergetics stimulate the new infrared approach for mechanistic investigation of any other protein function.
منابع مشابه
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تاریخ انتشار 2013